Review



orf3 mutant strain  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC orf3 mutant strain
    Orf3 Mutant Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/orf3+mutant+strain/Acinetobacter+baumannii%3B+2208/pmc02737869-25-30-40
    Average 99 stars, based on 3752 article reviews
    orf3 mutant strain - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Mutagenesis:

    Article Title: CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter baumannii
    Article Snippet: .. Etest susceptibility assays to quinolones, tetracyclines, aminoglycosides, and imipenem (Table ) showed identical MICs between ATCC 19606 and JVAB01, but a dramatic drop in chloramphenicol resistance was detected for the Orf3 mutant strain (a MIC value of >256 μg/ml for ATCC 19606 compared to 2 μg/ml for JVAB01). table ft1 table-wrap mode=article t1 TABLE 1. caption a4 MICs of antimicrobial agents in the investigated A. baumannii strains To check that the increased chloramphenicol susceptibility observed for the JVAB01 strain was in fact due to the lack of a functional Orf3 protein, a 1,971-bp PCR fragment containing the A. baumannii ATCC 19606 orf3 gene together with 520 bp upstream from the ATG codon was amplified by PCR and introduced into the EcoRI site of the shuttle vector pWH1266 ( 5 ), generating the plasmid pJV103. ..

    Article Title: CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter baumannii
    Article Snippet: .. Etest susceptibility assays to quinolones, tetracyclines, aminoglycosides, and imipenem (Table 1) showed identical MICs between ATCC 19606 and JVAB01, but a dramatic drop in chloramphenicol resistance was detected for the Orf3 mutant strain (a MIC value of 256 g/ml for ATCC 19606 compared to 2 g/ml for JVAB01). .. To check that the increased chloramphenicol susceptibility observed for the JVAB01 strain was in fact due to the lack of a functional Orf3 protein, a 1,971-bp PCR fragment containing the A. baumannii ATCC 19606 orf3 gene together with 520 bp upstream from the ATG codon was amplified by PCR and introduced into the EcoRI site of the shuttle vector pWH1266 (5), generating the plasmid pJV103.

    Functional Assay:

    Article Title: CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter baumannii
    Article Snippet: .. Etest susceptibility assays to quinolones, tetracyclines, aminoglycosides, and imipenem (Table ) showed identical MICs between ATCC 19606 and JVAB01, but a dramatic drop in chloramphenicol resistance was detected for the Orf3 mutant strain (a MIC value of >256 μg/ml for ATCC 19606 compared to 2 μg/ml for JVAB01). table ft1 table-wrap mode=article t1 TABLE 1. caption a4 MICs of antimicrobial agents in the investigated A. baumannii strains To check that the increased chloramphenicol susceptibility observed for the JVAB01 strain was in fact due to the lack of a functional Orf3 protein, a 1,971-bp PCR fragment containing the A. baumannii ATCC 19606 orf3 gene together with 520 bp upstream from the ATG codon was amplified by PCR and introduced into the EcoRI site of the shuttle vector pWH1266 ( 5 ), generating the plasmid pJV103. ..

    Polymerase Chain Reaction:

    Article Title: CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter baumannii
    Article Snippet: .. Etest susceptibility assays to quinolones, tetracyclines, aminoglycosides, and imipenem (Table ) showed identical MICs between ATCC 19606 and JVAB01, but a dramatic drop in chloramphenicol resistance was detected for the Orf3 mutant strain (a MIC value of >256 μg/ml for ATCC 19606 compared to 2 μg/ml for JVAB01). table ft1 table-wrap mode=article t1 TABLE 1. caption a4 MICs of antimicrobial agents in the investigated A. baumannii strains To check that the increased chloramphenicol susceptibility observed for the JVAB01 strain was in fact due to the lack of a functional Orf3 protein, a 1,971-bp PCR fragment containing the A. baumannii ATCC 19606 orf3 gene together with 520 bp upstream from the ATG codon was amplified by PCR and introduced into the EcoRI site of the shuttle vector pWH1266 ( 5 ), generating the plasmid pJV103. ..

    Amplification:

    Article Title: CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter baumannii
    Article Snippet: .. Etest susceptibility assays to quinolones, tetracyclines, aminoglycosides, and imipenem (Table ) showed identical MICs between ATCC 19606 and JVAB01, but a dramatic drop in chloramphenicol resistance was detected for the Orf3 mutant strain (a MIC value of >256 μg/ml for ATCC 19606 compared to 2 μg/ml for JVAB01). table ft1 table-wrap mode=article t1 TABLE 1. caption a4 MICs of antimicrobial agents in the investigated A. baumannii strains To check that the increased chloramphenicol susceptibility observed for the JVAB01 strain was in fact due to the lack of a functional Orf3 protein, a 1,971-bp PCR fragment containing the A. baumannii ATCC 19606 orf3 gene together with 520 bp upstream from the ATG codon was amplified by PCR and introduced into the EcoRI site of the shuttle vector pWH1266 ( 5 ), generating the plasmid pJV103. ..

    Plasmid Preparation:

    Article Title: CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter baumannii
    Article Snippet: .. Etest susceptibility assays to quinolones, tetracyclines, aminoglycosides, and imipenem (Table ) showed identical MICs between ATCC 19606 and JVAB01, but a dramatic drop in chloramphenicol resistance was detected for the Orf3 mutant strain (a MIC value of >256 μg/ml for ATCC 19606 compared to 2 μg/ml for JVAB01). table ft1 table-wrap mode=article t1 TABLE 1. caption a4 MICs of antimicrobial agents in the investigated A. baumannii strains To check that the increased chloramphenicol susceptibility observed for the JVAB01 strain was in fact due to the lack of a functional Orf3 protein, a 1,971-bp PCR fragment containing the A. baumannii ATCC 19606 orf3 gene together with 520 bp upstream from the ATG codon was amplified by PCR and introduced into the EcoRI site of the shuttle vector pWH1266 ( 5 ), generating the plasmid pJV103. ..



    Similar Products

    orf3 mutant strain  (ATCC)
    99
    ATCC orf3 mutant strain
    Orf3 Mutant Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/orf3+mutant+strain/Acinetobacter+baumannii%3B+2208/pmc02737869-25-30-40
    Average 99 stars, based on 1 article reviews
    orf3 mutant strain - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results